Product Name :
Phospho-EGFR (Y1068) Recombinant Rabbit Monoclonal Antibody [SD2055]
Predicted band size :
134 kDa
Observed band size :
170 kDa
Synonyms:
Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody Cell growth inhibiting protein 40 antibody Cell proliferation inducing protein 61 antibody EGF R antibody EGFR antibody EGFR_HUMAN antibody Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibody Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibody Epidermal growth factor receptor antibody erb-b2 receptor tyrosine kinase 1 antibody 展开
Function :
Epidermal growth factor mediates its effects on cell growth through its inter-action with a cell surface glycoprotein designated the EGF receptor. Binding of EGF or TGF alpha to the EGF receptor activates tyrosine-specific protein kinase activity intrinsic to the EGF receptor. The carboxy terminal tyrosine residues on EGFR, Tyr 1068 and Tyr 1173, are the major sites of autophosphorylation, which occurs as a result of EGF binding. Once activated, EGFR mediates the binding of the phosphotyrosine binding (PTB) domain of GRB2 through direct interactions with Tyr 1068 and Tyr 1086 and through indirect interactions with Tyr 1173 in the Ras signaling pathway. Tyr 1173 of EGFR also functions as a kinase substrate. Phosphorylation of Tyr 992, Tyr 1068 and Tyr 1086 is required for conformational change in the C-terminal tail of the EGF receptor.Formula of 10504-60-6
Antibody Type:
Recombinant Rabbit monoclonal Antibody
Immunogen :
Synthetic phospho-peptide corresponding to residues surrounding Tyr1068 of human EGFR.6-Bromo-3-chloroisoquinoline supplier
Species reactivity:
Human
Verify application:
WB, IF-Cell, IF-Tissue, IP, FC
Coupling :
unconjugated
Clone number:
SD2055
RRID:
AB_3070103
Form :
Liquid
Concentration :
1ug/ul
Storage instructions:
Store at +4℃ after thawing.PMID:33719535 Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer solution:
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Subtype:
IgG
Purification method :
Protein A affinity purified.
ELTSA :
Molecular weight :
Predicted band size: 134 kDa
Positive control :
HeLa cell lysate, HeLa treated with 100ng/mL EGF for 30 minutes cell lysate, A431, Hela, MCF-7.